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Título

OBESITY INFLUENCE ON BLADDER INFLAMMATION: A CYSTITIS MODEL

Resumo

Background: Recently the role of subclinical inflammation in obesity gained prominence. The association between obesity and chronic inflammation has been observed in several studies that show the relationship between increased morbidity and high Body Mass Index (BMI). This study aims to compare inflammatory pathways in obese and non-obese mice after exposure to a pro-inflammatory carcinogen as a cystitis model.
Methods: We divided 16 females, 7 weeks old mice into two groups: 1) CONTROL: standard diet and 2) OBESE: high-fat diet. Both groups underwent a protocol for MNU carcinogen bladder instillation. The bladder was analyzed by histopathology, western blotting, and immunohistochemistry.
Results: Most OBESE mice (62.5%) presented moderate chronic active inflammation in the submucosa and mild submucosal hyalinization and 37.5% presented moderate chronic active inflammation in the submucosa and diffuse muscle layers. Most CONTROL mice (75%) had no histological alteration in bladder tissue and 25% had hyalinization of the submucosal layer. Obese mice had a high expression of inflammatory proteins inflammatory pathway (JNK, IKKβ / IkB / NFkB) and higher apoptotic and inflammatory indexes.
Conclusion: Cystitis might exacerbate in obese mice which represents a chronic inflammatory state.

Palavras Chave ( separado por ; )

inflammation, cystitis, MNU, mice, obesity